Induction of a colitogenic phenotype in Th1-like cells depends on interleukin-23 receptor signaling [in vivo]

Th1 cells were adoptively transferred into RAG1 KO mice from either wildtype (Il23rwt/eGFP) or KO (Il23reGFP/eGFP) donors. At the peak of disease in recipients of wildtype cells, the animals were euthanized and intestinal T cells were isolated from the chosen recipients of both wildtype and KO cells for 10x massively parallel single-cell RNA-sequencing. From both recipients of wildtype (Il23rwt/eGFP) or KO (Il23reGFP/eGFP) Th1 cells the following tissues were isolated for single-cell RNA sequencing: spleen, small intestine lamina propria (LPL), colon LPL and colon intraepithelial lymphocytes (IEL).