Efficiency of co-transfection of hLF BAC and pCEIN by microinjection.
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*After approximately 20 days, the positive colonies (expressing GFP and Neor) were screened by G418 and confirmed by the expression of GFP.?After the GFP and Neor positive colonies were selected and expanded, DNA extraction was performed using some cells from each colony, and the integrated cells were determined by PCR using primer P1, P2 and P3.
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2015-12-02
