DNA methylation analysis of BeWo cells stimulated by tumor necrosis factor-alpha and insulin-like growth factor-I. DNA methylation analysis of BeWo cells stimulated by tumor necrosis factor-alpha and insulin-like growth factor-I

Cellular functions of trophoblasts are regulated by various cytokines and growth factors. We previously reported the synergistic effects of tumor necrosis factor-α (TNF-α) and insulin-like growth factor-I (IGF-I) on BeWo cells proliferation. We performed DNA methylation array to elucidate the epigenetic regulation involved in the cross-talk of TNF-α and IGF-I in BeWo cells. We identified general increase in DNA methylation by co-stimulation of TNF-α and IGF-I. Total of 83474 differentially methylated CpG sites were identified in BeWo cells stimulated by IGF-I and high TNF-α. By correlating with the results of expression array, NFKBIE, C3 and GP1BA were identified as differentially methylated genes with significant expression change. Overall design: Bisulphite converted DNA from 12 samples of BeWo cells were hybridised to Illumina Infinium MethylationEPIC Beadchip