N-terminal acetylation determination of PEXEL proteins

We have knocked out a putative N-acetyl transferase, Pf3D7_1437000, from the malaria parasite plasmodium falciparum. Pf3D7_1437000 resides in the ER. Proteins in the Plasmodium falciparum secretory pathway are known to be extensively N-terminally acetylated. We have isolated two such proteins, HRP2 and HRP3, from the WT and KO parasites compared there N terminal acetylation status.