Use of Human Adult Stem Cell-Derived Organoid Cultures to Model Enterohepatic Circulation [Ileal organoids]

Organ to organ interactions have not yet been modeled with human organoid cultures. This work demonstrates the coupling of intestinal and liver organoids recapitulates aspects of enterohepatic bile signaling. The integration of organoids can be used to probe intra-organ interactions. Total RNA-Seq studies were performed in human ileal organoids to examine expression of bile acid signaling molecules. Ileal organoids from two independent individuals were used for total RNA sequencing.