Primers and probe details showing sequences, target taxa and fragment sizes.
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https://figshare.com/articles/dataset/_Primers_and_probe_details_showing_sequences_target_taxa_and_fragment_sizes_/254946
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Fragment sizes are given in base pairs including primers. All primers were designed for this study and amplify part of the Cytochrome b (cyt-b) gene. All regular PCRs were performed at 50°C annealing temperature and all qPCRs at 60°C annealing temperature. Probes are Minor Groove Binding (MGB) probes and have the modifications; 5′: 6-Fam (D-L-Probe), 3′: BHQ-1.
创建时间：
2012-08-29
