Flow cytometric analysis of neutrophil populations present in orthotopic KCKO cancers

Chronic inflammatory milieu in the tumor microenvironment (TME) leads to the recruitment and differentiation of myeloid-derived suppressor cells (MDSCs). Polymorphonuclear (PMN)-MDSCs, which are phenotypically and morphologically defined as a subset of neutrophils, cause major immune suppression in the TME, posing a significant challenge in the development of effective immunotherapies. Despite recent advances in our understanding of PMN-MDSC functions, the mechanism that gives rise to immunosuppressive neutrophils within the TME remains elusive. Both in vivo and in vitro, newly recruited neutrophils into the tumor sites remained activated and highly motile for several days and developed immunosuppressive phenotypes, as indicated by increased arginase 1 (Arg1) and dcTrail-R1 expression and suppressed anti-cancer CD8 T cell cytotoxicity. The strong suppressive function was successfully recapitulated by incubating naïve neutrophils with cancer cell culture supernatant in vitro. Cancer meta..., This data set contains raw fcs flow cytometric data from in vivo experiments on naive and tumor bearing mice. , , # Flow Cytometric Data set

[https://doi.org/10.5061/dryad.866t1g209](https://doi.org/10.5061/dryad.866t1g209)

Dataset contains raw fcs files from *in vivo* experiments described in the manuscript. 

## Description of the data and file structure

Raw fcs files of tumor digestion. All markers are labeled in the dataset

***Name of dataset:*** (number after the name denotes biological replicate)

*N_BM* (Bone marrow isolated from naive mice)

*T_BM* (Bone marrow isolated from tumor bearing mice)

*N_SPL* (Spleen isolated from naive mice)

*T_SPL* (Spleen isolated from tumor bearing mice)

*Tumor* (KCKO tumor)