Enzymatic parameters of cofactor interactions with lid domain mutants.

ATPase activities were determined in standard buffer as described in the Materials and Methods section. DNJ-13 stimulation or BAG-1 inhibition were not observed in some experiments (denoted by “n.d.”). Consequently an apparent KD cannot be deduced. The semi-quantitative value “tight” points to the fact that in the respective experiment, quantitative binding appeared substoichiometric. Consequently, no reasonable data fitting can be performed, using the normal absorption isotherm. KD denotes the apparent affinity. The errors represent standard deviations of three independent experiments.