RNA sequencing using 2D cells and organoids derived from canine bladder cancer and healthy canine bladder epithelial cells.

In human and dogs, bladder cancer (BC) is the most common neoplasm affecting the urinary tract. Dog BC resembles human muscle-invasive BC in histopathological characteristics and gene expression profiles, and could be an important research model for this disease. However dog BC organoids had never been produced. Therefore we aimed to generate dog BC organoids using urine samples.We performed RNA sequencing using 2D cell, and organoids derived from canine bladder cancer and healthy canine bladder epithelial cells.