Expression data from Drosophila histone methyltransferase mutants. Drosophila melanogaster

H3K9 methylation is a mark of inactive chromatin. In D.melanogaster, three enzymes (SU(VAR)3-9, EGG, and dG9A) are responsible for creating the H3K9 methyl mark. We have investigated the effects of loss of SU(VAR)3-9 and EGG on gene expression in adult heads using microarray analysis. Keywords: Expression profiling by microarray Overall design: Heads from adults flies were used for RNA extraction and hybridization on Affymetrix microarrays. Four genotypes were used: yw; +/CyO, yw; egg235/CyO, yw; +/CyO; Su(var)3-906; yw; egg235/CyO; Su(var)3-906. For each genotype, three biological replicates were analyzed. For the yw; +/CyO control, an additional technical replicate (same RNA sample processed twice) is included. While source files for both technical replicates are included, only one was used for the final analysis.