Expression data from graft infiltrating macrophages treated with mTORi-HDL nanobiologics. Expression data from graft infiltrating macrophages treated with mTORi-HDL nanobiologics

Inducing graft acceptance without chronic immunosuppression remains an elusive goal in organ transplantation. Recent data demonstrate that non-self recognition by graft infiltrating macrophages initiates transplant rejection. Using an experimental transplantation mouse model, we isolated graft-infiltrating macrophages from two transplantation settings: untreated rejecting mice and treated with mTORi-HDL nanobiologics. We used microarrays to detail the global programme of gene expression underlying macrophage dependent organ transplant rejection and identified distinct classes of up-regulated genes during this process, which are down-regulated following tolerogenic treatment with mTORi-HDL nanobiologics. Overall design: BALB/c donor hearts were transplanted into fully allogeneic C57BL/6 mice. Transplant recipients were either left untreated (Control) and rejected their allografts or were treated with mTORi-HDL nanobiologics (Sample) on days 0, 2, and 5 post-transplantation. Donor heart allografts were isolated on day 6 post-transplantation and graft-infiltrating macrophages were isolated by fluorescence-activated cell sorting (FACS) for microarray analysis.