mRNA expression data from RalGAPβ-deficient human pancreatic ductal adenocarcinoma cells (MIA PaCa-2), MIA PaCa-2 parental cells, and MIA PaCa-2 control cells

Pancreatic ductal adenocarcinoma, caused by activating mutation in K-Ras, is an aggressive malignancy due to its early invasion and matastasis. Ral GTPases, negatively regulated by RalGAP, are activated downstream of Ras and play a crucial role in development and progression of pancreatic ductal adenocarcinoma. However, the underlying mechanisms remain unclear. We used microarrays to detail the global programme of gene expression underlying the human pancreatic ductal adenocarcinoma cell line, MIA PaCa-2 with RalGAPβ deficiency or not, and identified distinct classes of Ral activation-related mRNA. We used two clonal RalGAPβ-deficient human pancreatic ductal adenocarcinoma cell lines of MIA PaCa-2 (KO1 and KO2) established by the CRISPR-Cas9 system, and each was compared with parental cells (WT) and control cells (Con) using microarray analysis. WT refers to parental MIA PaCa-2 cells without any treatments. Con refers to a clonal MIA PaCa-2 cell line obtained through the same RalGAPβ CRISPR-Cas9 treatment but also no indel mutation in the RalGAPβ genomic region. These cells were used for RNA extraction and hybridization on Affymetrix microarrays.