Supplementary materials for the article reporting Pacific Oyster Nidovirus 1 (PONV1)

Here, we provide supplementary materials, including phylogenetic trees, sequences, and relevant codes, for the article (Zhong et al. 2025; https://doi.org/10.1073/pnas.2426923122) reporting Pacific Oyster Nidovirus 1 (PONV1), detected in farms where Pacific oysters experiencing mass mortality events in British Columbia, Canada.

Contents of the sequence file:Rdrp_containing_contig_unique_vOTU_189.fasta
Representative nucleotide sequences of 189 RdRp-containing RNA virus operational taxonomic units (vOTUs) identified from the Pacific oyster samples (ENA accession numbers: ERR13334386 to ERR13334481).PONV1_segment_1.fasta
Nucleotide sequences of segment 1 from PONV1.
NCBI accession number: PQ030832.PONV1_segment_2.fasta
Nucleotide sequences of segment 2 from PONV1.
NCBI accession number: PV579160.PONV1-like_virus_segment_1.fasta
Nucleotide sequences of segment 1 from PONV1-like virus 1 through 15.
NCBI accession numbers: BK068944 to BK068958.PONV1-like_virus_segment_2.fasta
Nucleotide sequences of segment 2 from PONV1-like virus 1 through 15.
NCBI accession numbers: PV928314 to PV928328.

Reference:Zhong KX, Chan AM, Miller KM, Saunders R, and Suttle CA (2025) Evolutionarily divergent nidovirus with an exceptionally large genome identified in Pacific oysters undergoing mass mortality. Proceedings of the National Academy of Sciences USA, 122: e2426923122. https://doi.org/10.1073/pnas.2426923122