Number of MT-ND1 and B2M real time PCR plates required to detect a specific relative difference in mtDNA copy number between two homogenate skeletal muscle DNA samples for a given number of replicate sample wells.

Each cell defines the number of plates (n) and actual power (P; minimum power 0.8 at α = 0.05) for a two sample t-test to detect, with the specified number of sample replicates per plate, a given percentage difference in mtDNA copy number. Estimates are calculated at high (approximate CqMT-ND1 of 19 ([DNA]PCR = 20 pg/µl) and CqB2M of 27 ([DNA]PCR = 0.6 ng/µl)), moderate (approximate CqMT-ND1 of 24 ([DNA]PCR = 0.6 pg/µl) and CqB2M of 30 ([DNA]PCR = 0.1 ng/µl)) and low (approximate CqMT-ND1 of 25 ([DNA]PCR = 0.3 pg/µl) and CqB2M of 33 ([DNA]PCR = 10 pg/µl)) PCR DNA concentration levels.Number of MT-ND1 and B2M real time PCR plates required to detect a specific relative difference in mtDNA copy number between two homogenate skeletal muscle DNA samples for a given number of replicate sample wells.