Enhancer analysis of rat cardiac myocytes and fibroblasts reveals a collaborative control by transcription factor families [ChIP-Seq]

We used a differential open chromatin approach, coupled with active enhancer mark ,transcriptomic and computational TFs binding analysis to map cell-type-specific active enhancers in cardiac fibroblasts and cardiomyocytes, and outline the TFs that control them. We identified Tead, Sox9, Smad, Tcf, Meis, Rbpj, and Runx1 as the main cardiac fibroblasts TF groups. Our analysis shows that in cardiomyocytes and cardiac fibroblasts, distal enhancers, containing concentrated combinatorial clusters of multiple tissue expressed TFs recognition motifs, are combinatorically clustered around tissue specific genes.本研究采用差异开放染色质分析方法（differential open chromatin approach），联合活性增强子标记（active enhancer mark）、转录组学及转录因子结合计算分析，对心脏成纤维细胞（cardiac fibroblasts）与心肌细胞（cardiomyocytes）中的细胞类型特异性活性增强子进行定位，并阐明调控此类增强子的转录因子（Transcription Factor, TF）。我们鉴定出Tead、Sox9、Smad、Tcf、Meis、Rbpj及Runx1为心脏成纤维细胞的主要转录因子组。分析结果表明，在心肌细胞与心脏成纤维细胞内，携带有多种组织特异性表达的转录因子识别基序密集组合簇的远端增强子，会在组织特异性基因周围形成组合式聚集的调控结构。