Inhibition of L-type amino acid transporter 1 induces disruption of cancer amino acid homeostasis with altered cellular signals

This study characterized the changes of phosphorylation signaling cascades induced by LAT1 inhibition in three cell lines of biliary tract cancer treated with LAT1 specific inhibitor JPH203 for 15 and 30 min. Immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometry with MS2-based quantitation of tandem mass tag were utilized.