Angiogenic, apoptotic and autophagic profiling of chronic myeloid leukaemia patients’ platelets ex vivo before and after treatment with Imatinib

The data presented here was to
determine the angiogenic, apoptotic, and autophagic profiles of chronic myeloid
leukaemia patients ex vivo on
platelets before and after treatment with Imatinib. Platelet counts, viability and activation were determined by human cluster of
differentiation (CD) 41 and -62 markers. The possible occurrence of apoptosis
and autophagy was measured by means of flow cytometry and western blot analysis
via Annexin V-fluorescein isothiocyanate (FITC), caspase 3, autophagy related gene
5 (Atg5) and light chain I and II (LC3) markers. Angiogenesis expression was
determined through vascular endothelial growth factor (VEGF) and transforming
growth factor β (TGFβ) markers.

本数据集旨在确定慢性髓性白血病病人在体外血小板上的血管生成、细胞凋亡和自噬特征，分别在治疗伊马替尼前后的情况。通过检测人类CD41和CD62标记物，确定了血小板计数、活性和活化情况。通过流式细胞术和Annexin V-异硫氰酸荧光素（FITC）、caspase 3、自噬相关基因5（Atg5）以及轻链I和II（LC3）标记物，测量了细胞凋亡和自噬的可能发生。血管生成表达通过血管内皮生长因子（VEGF）和转化生长因子β（TGFβ）标记物进行确定。