Identification and diagnosis of possible mutations in human P-53 lymphocytes

Data description It was discovered in the last few years that the production of some percentage of mutant p-53 proteins, with the increased stability in type B lymphocytes, leads to the carcinogenesis process. In the full physical examination of patients diagnosed with Chronic Lymphocytic Leukemia were encompassed all patients which have been admitted to hospital with symptoms and clinical features. Male results: Protein concentration in p-53 / µg / dL: 20, 15, 18, 40, 10, 12, 14, 60, 30, 10, 13, 15, 5, 10, 15, 12. Women's results; Protein concentration p-53 / µg / dL: 140, 30, 13, 10. Normal values of normal cell lines on equipment: ELISA = 0.25-0.5 µg / dL, or 2.5- 5ng/mL, [Figure 5]. Very high pathological values in the 3 cases of p-53 were calculated in 2 men in the value of 60 µg / dl, respectively at 40 µg / dl, and in the case of females it was calculated in the amount of 140 µg / dl, the frequency chronic lymphocytic leukemia with transformation into Diffuse Large Lymphoma, (DLL). Statistical interpretations: the concentration of p-53 protein, in the 17 cases with p-53 protein expression, after excluding the 3 out-line cases present in the study, was calculated at the average value of 16.76µg / dl, with STDEV = 8.35, CV = 0.5% and the probability index (NORMDIST) p, was calculated in the value of p = 0.034. The concentration of p-53 protein, in the 17 cases with p-53 protein expression, after excluding the 3 out-line cases present in the study, was calculated at the average value of 16.76µg / dL. In the CLL stage III/IV (n = 3 patients (Table 1), the percentage of p53 isoform protein with elevated values was present in 15% (3 of 20 cases), such as 2 Men = 50μg / dL and 60μg / dl, respectively and 1 Female = 140μg /dL, p value 0.034; Hematological parameters in peripheral blood were changed with the Leucocytes leukocyte number = 250-500 x 10 3 /dL, Hb = 8.6g / dl, Thrombocytosis = 45x10 3 / dL, Lymphocytes in peripheral blood= 85-90%, ( p value = 0.05). In some international studies, the immunological characteristics of patients with CLL having p-53 protein positive, were measured by immunohistochemistry, (IHC). In the second part of the study CLL on the stage I/II, (n=47 patients), the P-53 protein isoform concentration in reactive Lymphocyte B, the average p-53 protein concentration in CLL was on average 47 U/m with 16.7% samples studied, (7 out of 42 cases), Hematological parameters in peripheral blood, Leucocyte number = 35x10³ / dl, Hb = 12,2g / d, Thrombocyte = 140 x x10³ / dL, Lymphocytes in peripheral blood = 75-80%. The average p-53 protein concentration in CLL was on average 47 U/m, in CLL on the stage III/IV (n = 140 patients] and percentage of p53 isoform varied from 7-32%.; Hematological parameters in peripheral blood where changed in Leukocytes number = 350 x10³ / dL, Hb = 10,8g / dL, Thrombocytes = 80 x 10³ / dL, Lymphocytes in peripheral blood = 80-90%; Percent p-53 positive was in percent of 15% 10.数据集描述 近数年研究发现，一定比例的突变型p53蛋白（mutant p-53）在B淋巴细胞中稳定性提升，可诱发癌变进程。本研究对确诊为慢性淋巴细胞白血病（Chronic Lymphocytic Leukemia, CLL）的患者进行全面体格检查时，纳入了所有因相关症状与临床特征入院的患者。 男性检测结果：p53蛋白浓度/μg/dL：20、15、18、40、10、12、14、60、30、10、13、15、5、10、15、12。女性检测结果：p53蛋白浓度/μg/dL：140、30、13、10。本设备检测正常细胞系的参考正常值为：酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）0.25~0.5 μg/dL，或2.5~5 ng/mL，详见图5（Figure 5）。3例极高病理值的p53蛋白检测结果分别为：2名男性分别为60 μg/dL、40 μg/dL，1名女性为140 μg/dL；该类病例多伴随慢性淋巴细胞白血病向弥漫大淋巴细胞瘤（Diffuse Large Lymphoma, DLL）转化。 统计分析：剔除本研究中3例异常值后，17例存在p53蛋白表达的病例其p53蛋白平均浓度为16.76 μg/dL，标准差（STDEV）为8.35，变异系数（CV）为0.5%，正态分布概率指数（NORMDIST）p值为0.034。 剔除本研究中3例异常值后，17例存在p53蛋白表达的病例其p53蛋白平均浓度为16.76 μg/dL。针对Ⅲ/Ⅳ期慢性淋巴细胞白血病（CLL）患者（n=3，见表1），p53亚型蛋白表达升高的比例为15%（20例中占3例），分别为2名男性（50 μg/dL、60 μg/dL）与1名女性（140 μg/dL），p值为0.034；外周血液学参数发生改变：白细胞计数250~500×10³/dL，血红蛋白（Hb）8.6 g/dL，血小板增多症（Thrombocytosis）表现为血小板计数45×10³/dL，外周血淋巴细胞占比85%~90%（p值=0.05）。 部分国际研究中，采用免疫组织化学法（Immunohistochemistry, IHC）检测p53蛋白阳性的CLL患者的免疫学特征。本研究第二部分纳入Ⅰ/Ⅱ期CLL患者（n=47），针对反应性B淋巴细胞中的p53蛋白亚型浓度进行检测：CLL患者的p53蛋白平均浓度为47 U/m，其中16.7%的研究样本（42例中7例）呈阳性；外周血液学参数为：白细胞计数35×10³/dL，血红蛋白12.2 g/dL，血小板计数140×10³/dL，外周血淋巴细胞占比75%~80%。 Ⅲ/Ⅳ期CLL患者（n=140）的p53蛋白平均浓度为47 U/m，p53亚型阳性率为7%~32%；外周血液学参数改变为：白细胞计数350×10³/dL，血红蛋白10.8 g/dL，血小板计数80×10³/dL，外周血淋巴细胞占比80%~90%；p53阳性率为15%。