RNA-seq and small RNA-seq of cattle jejunum tissue

We applied whole transcriptome sequencing analysis to examine the jejunum tissue in nine Chinese Holstein cows. We screened out differentially expressed genes, lncRNAs, and miRNAs of three comparison groups, i.e, clinical vs. healthy, subclinical vs. healthy, and clinical vs. subclinical, and identified ceRNA pairs based on the mRNA, lncRNA, and miRNA data. Through integrating these DEGs with functional annotation, previously reported QTLs, and GWAS results, we proposed some genes, lncRNAs, miRNAs, and ceRNA pairs implying their potential associations with susceptibility to MAP infection.