Chemical Perturbation of CDK9 and RUVBL2 Reveals Insights on Regulation of the Circadian Phase

We report the CRY1 ChIPseq data. Mice were intraperitoneally (I.P.) injected with DRB at CT02 (CT: circadian time). DRB (5,6-dichlorobenzimidazone-1-ß-D-ribofuranoside) is a well-known inhibitor of the transcription factor CDK9 which has crucial roles in the RNA polymerase II transcriptional pausing. The mouse liver was collected at 0, 2 and 4hr after treatment. Then, the samples were processed as we previously reported (Koike et al, 2012, Science). This study provides the evidence that DRB regulates the CRY1 binding dynamics. Overall design: Examination of the DRB effects on the Cry1 biding. DMSO(0hr)_DMSO(2hr)_DMSO(4hr)_DRB(0hr)_DRB(2hr)_DRB(4hr)_and_INPUT