Complete genome sequence of Picosynechococcus sp. strain NKBG15041c, a fast-growing marine cyanobacterium

Here, we report the complete genome sequence of Picosynechococcus sp. strain NKBG15041c, which was isolated from coastal seawater in Okinawa prefecture, Japan. Additionally, we determined the complete sequences of three associated plasmids. The genomic DNA was sequenced using both Illumina and PacBio platforms. Genome assembly, integrating PacBio long reads and Illumina short reads, was performed using B-assembler. The longest circular contig represents the complete genome of P. NKBG1504c, comprising 2,845,435 bp with a G+C content of 49.8%. The three circular contigs showed high homology with plasmids from Synechococcus sp. NIES-970., , # Complete genome sequence of Picosynechococcus sp. strain NKBG15041c, a fast-growing marine cyanobacterium

This Readme file lists the raw sequence files of Illumina short reads and PacBio Long reads for the genome of *Picosynechococcus* sp. strain NKBG1504c.

Dataset DOI: [10.5061/dryad.wdbrv161k](10.5061/dryad.wdbrv161k)

## Description of the data and file structure

*P*. NKBG15041c was cultivated in BG11 medium supplemented with 3% NaCl under continuous illumination (50 µmol photons m⁻² s⁻¹) at 30 °C with shaking at 100 rpm. Cells were grown until the OD₆₆₀ reached 0.4, and then harvested by centrifugation at 8,000×g for 10 minutes at 4℃. Genomic DNA was extracted from bacterial pellets using ISOIL for Bead Beating (NIPPON GENE) according to the manufacturer’s instructions. Following RNase treatment for 30 minutes at 4 °C, the DNA was purified by phenol-chloroform extraction and ethanol precipitation.

For Illumina sequencing, 500 ng of genomic DNA was fragmented to a target size o...,