ZNF384 fusion oncoproteins drive lineage aberrancy in acute leukemia [ChIP-seq]

We report changes in chromatin occupancy dependent on TCF3-ZNF384 splicing isoform usage. The isoform including exon 8, which encodes 2 zinc finger domains, has increased binding near genes important for nomal hematopoiesis. We report no global changes in H3K27Ac upon expression of ZNF384 fusions but 47 loci with increased H3K27Ac. Overall design: 4 replicates of HA-tagged fusion proteins expressed in ARF-null pre-B cells or 2 replicates of H3K27Ac in fusion-expressing or wildtype expressing cells.