Next Generation Sequencing Quantitative Analysis of exosomes of hepatocellular carcinoma patients and healthy people. Next Generation Sequencing Quantitative Analysis of exosomes of hepatocellular carcinoma patients and healthy people

We report the application of miRNAs analysis in exosomes based on second-generation high-throughput sequencing. Plasma samples from 3 hepatocellular carcinoma patients and 3 healthy controls were collected, and exosomes were isolated by ultra-centrifugation. MiRNAs in exosomes were then extracted and high-throughput sequencing was performed.By comparing the sequencing data, we screened out differentially expressed miRNAs and validated them with qRT-PCR in exosome samples, establishing and verifying the diagnostic panel of hepatocellular carcinoma composed of miR-212-5p, miR-519b-3p, miR-1248 and miR-1250-5p. Overall design: Plasma exosome miRNAs from 3 hepatocellular carcinoma patients and 3 healthy volunteers