The effect of gut microbiota in patients with uremia on nutritional status and micro-inflammation of rat

Objective To explore the effects of intestinal flora in uremic patients on nutritional status and microinflammatory response in rats. Method Twenty-four SD male rats were randomly divided into 3 groups: control group, uremia group, healthy group. After 3 weeks, the intestinal flora destruction model of SD rats was established. Four non-dialysis patients with chronic kidney disease stage 5 ( CKD5 ) and four healthy people were selected, and their feces were collected. After treatment, they were poured into the intestinal tract of SD male rats in uremia group and healthy group respectively. After 2 weeks, the cervical spine was cut off and the blood was taken and the fat around the epididymis and kidney in the abdominal cavity was retained. Result After 3 weeks of oral administration of compound antibiotics, the intestinal flora of SD rats changed significantly, which showed that the diversity of intestinal flora decreased significantly. There are differences in intestinal flora between uremic patients and healthy people. The body weight, albumin, body fat ratio and cholesterol of male SD rats in uremia group were lower than those in healthy male SD rats (P < 0.05). The levels of interleukin-6 and C-reactive protein in uremia group were higher than those in healthy group (P < 0.05). Conclusion Uremia patients have intestinal flora disorders. Fecal implantation in uremic patients can change the nutritional status of rats and affect the inflammatory response of rats