Gene expression pattern of primed human neutrophils

Neutrophilic granulocytes change their functionality and phenotype in response to cytokines. This adaptation to the micro-milieu, also called priming, is eminent for an orchestrated defense against infection. However, in chronic inflammation, this process may be accompanied by a steady maintenance of inflammation and tissue destruction. To explore this in more detail, neutrophilic granulocytes were obtained from the peripheral blood of healthy adult donors and primed with cytokines in vitro. The gene expression profile of these cells was compared to resting cells using nCounter technology. Total RNA was isolated from 10.000 resting or primed human neutrophils using MagNA pure isolation according to the manufacturer's instructions. Three biological replicates were inlcuded.