Staphylococcus aureus Raw sequence reads

In this study, antibiotic resistant S. aureus strains were selected by serial passaging of S. aureus SG511 Berlin (NZ_CP076660.1). S. aureus SG511 was incubated with increasing concentrations of the polyketide antibiotic cervimycin, to generate cervimycin resistant mutants (CmR strains). Two compounds of the antibiotic complex were used, cervimycin C and cervimycin D. Cervimycin susceptible suppressor mutants were generated by passaging of the CmR strains in absence of cervimycin for several times. In total, nine cervimycin resistant strains and their respective suppressor mutants were isolated and whole genome sequencing was performed, using Illumina technologies. Mutations occurred in the essential histidine kinase WalK, the Clp protease proteolytic subunit ClpP or the Clp ATPase ClpC, and the heat shock protein DnaK. Allelic exchange experiments were performed to separate identified mutations from each other and evaluate individual effects on S. aureus. Thus, the I29F amino acid exchange in ClpP (identified in CmR-01, CmR-02 and CmR-03) was introduced into S. aureus SG511. On, the other hand, this clpP mutation was reverted in CmR-01 (ClpP-F29I), to gain a WalK single mutant, S. aureus WalK-A243V.