gRNA_Cas9_validation
收藏NIAID Data Ecosystem2026-03-12 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/ERP003672
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资源简介：
gRNA/Cas9 system is becoming more general tools for genome modification in mammalian cells, such as in human 293T cells, K562 cells, induced pluripotent stem (iPS) cells and embryonic stem (ES) cells. The efficiencies of double-strand break (DSB) introduction by gRNA/Cas9 are key for the successful engineering of the cell genome. To use gRNA/Cas9 system, we need to measure "cutting" efficiency by a precise method, which can be adapted in a high-throughput manner. In this study, we explore the possibility of MiSeq sequencing for the "cutting" efficinecy measurement. We expect that one run of MiSeq should be able to analyze efficiency of more than 12 gRNA/Cas9
创建时间：
2021-02-04
