Primer used to amplify VJα and VDJβ fragments of all cTCR constructs generated in this study.
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Antigen specificity and HLA-restriction of the respective TCR is given where known. aT cell clones 5C/169 and 2C/406 express an identical TCR and are derived from the same MLTC. bThe sequence 5’ of the BsaI restriction site of primer BsaI_BC44.22_VDJβ.rev has been modified to prevent hairpin formation. Template during PCR was either plasmid DNA or RT-cDNA derived from clonal T cells. nd = not determined. (XLSX)
创建时间:
2020-09-09



