Transfer of heterologous SS<sub>OM</sub>-mCherry reporter in <i>M. xanthus</i> swarm requires TraA.
收藏NIAID Data Ecosystem2026-03-07 收录
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Nonmotile SSOM-mCherry donors (DW1411 or DW1412) were mixed 1∶3 with A-motile GFP labeled recipients (DW1414 or DW1416). After 1 day incubation on TPM agarose pad A-motile recipients readily swarm out from the inoculum spot. Column micrographs were of identical fields taken under phase contrast and GFP or mCherry fluorescence (20× objective). Indicated isogenic strains contain traA+ or traA::km alleles. In panels F and I arrows indicate nonmotile donor cells that were pushed or dragged to the swarm edge [6]. Table S2 lists strain genotypes.
创建时间:
2016-02-24



