Screening_of_Plasmodium_berghei_artificial_chromosome_libraries_by_Transposon_Directed_Insertion_Site_Sequencing__TraDIS_. Screening_of_Plasmodium_berghei_artificial_chromosome_libraries_by_Transposon_Directed_Insertion_Site_Sequencing__TraDIS_

We have recently generated an arrayed P. berghei artificial chromosome genomic (6-9 kb insert-size) library containing 7043 clones successfully mapped to the P. berghei genome by paired-end capillary sequencing. This library will act as the starting point for systematic screening of potential drug resistance genes. Pools of P. berghei artificial chromosome vectors will be transfected under pressure of an array of known and putative antimalarial drugs. Enrichment of artificial chromosome genomic clones carrying genes which selective amplification provides protection against a specific drug is expected. TraDIS is to be utilised to identify and quantify artificial chromosome clones as a read-out following drug selection. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/