Comparative gene expression profiling of serum-free and FBS treated colorectal TIC cultures

To induce a differentiated phenotype, primary colorectal TIC cultures were grown in 10% FBS containing conditions. To analyze gene expression changes upon induction of a differentiated phenotype, total RNA of cells cultured in FBS containing conditions and control cells cultured under serum-free conditions was isolated and comparative gene expression profiling was performed. RNA was obtained from five different patient derived spheroid cultures grown in serum-free or 10% FBS containing culture medium.