Optimisation of the extraction methods for proteomics analysis of three narrow-leafed lupin cultivars

In this study the proteome composition of three narrow-leafed lupin varieties (Tanjil, Unicrop, and P27255) resulted form four extraction methods was studied using discovery proteomics, this was followed by targeted quantitative analysis of the major lupin seed storage proteins known as conglutins.\nThe collection includes the following data:\n1- The information-dependent acquisition mass spectrometry (IDA-MS) data acquired on acquired on TripleTOF 6600 (SCIEX) mass\n2- ProteinPilot Reports for the IDA data which includes information about the data quality and protein and peptide identifications\n3-multiple reaction monitoring-mass spectrometry (LC-MRM-MS) data acquired on a 6500 QTRAP mass spectrometer (SCIEX)\n\nLineage: Proteome data were acquired using a liquid chromatograph-mass spectrometer (Eksigent nanoLC 415 - SCIEX 6600 QqTOF). Samples were separated using a C18 chromatography column and the eluting peptides measured by the mass spectrometer as mass-to-charge ratios. The mass spectrometer was operated in information-dependant acquisition mode whereby the machine surveys precursor ions and then selects mass-to-charge ratios for fragmentation in repeating cycles until the liquid chromatography program has completed.