Expression data from PrimeView human gene expression array

Gene expression in CAL-27 cells infected with shCtrl and shSCUBE3 was detected with a PrimeView human gene expression array. We performed Affymetrix Human Gene Chip Prime View analysis on shCtrl and shSCUBE3 CAL-27 cells. Of the 931 differentially expressed genes (DEGs) found in shCtrl and shSCUBE3 CAL-27 cells, 353 were up-regulated and 578 were down-regulated based on the threshold of absolute fold change ≥ 1.3 and FDR < 0.05 The total RNA of CAL-27 cells infected with shCtrl and shSCUBE3 was extracted. The RNA sequencing was performed with Affymetrix human GeneChip PrimeView and the outcomes were scanned by Affymetrix Scanner 3000. The statistical significance of raw data was completed by a Welch t-test with Benjamini-Hochberg FDR (|Fold Change| ≥ 1.3 and FDR < 0.05 as significant).