Responders to immune checkpoint therapy display early clonal expansion of tumour-infiltrating lymphocytes

Using murine models that exclude variation in host genetics, environmental factors and tumour mutation burden, limiting variation between animals to naturally diverse TCRß repertoires, we applied bulk TCRseq to study TCRß repertoire dynamics in ICT responders and non-responders Overall design: Bulk TCRßseq of tumor infiltrating lymphocytes at different timepoints prior to and during anti-CTLA-4 and anti-PD-L1 treatment, responders and non-responders. Each sample represents an individual biological replicate (i.e. no duplicate samples from the same mouse).