RNAseq of subepithelial connective tissue grafts obtained at different location and depth in the human palate

Aim: Identify set of genes, whose expression is dependent on the graft location in the patient's palate (anterior vs. posterior) and depth of harvest (superficial vs. deep). The mRNA-Seq contains 64 human samples. The samples represent connective tissue grafts (CTGs) harvested at different locations (anterior and posterior) and different depths (superficial and deep) in the patient's palate. Thus, we have 4 groups with 16 samples per group: asCTGs (anterior superficial) – 16 samples adCTGs (anterior deep) – 16 samples psCTGs (posterior superficial) – 16 samples pdCTGs (posterior deep) – 16 samples