Raw sequencing data and ngsfilters for snow track eDNA samples
收藏Mendeley Data2024-04-13 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.9kd51c5q1
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Snow tracks of brown bear, lynx and wolf were collected opportunistically during winter in 2019, 2020 and 2022 in the Slovenian Alps and Dinaric Mountains (seven brown bear samples and nine lynx samples) and in the French Alps (ten wolf samples). For genotyping of each species, we used a set of STR markers designed for optimal multiplex amplification and HTS genotyping. The brown bear set includes 13 STR recently described and used for individual profiling from fecal DNA, with the addition of a sex-specific marker. For wolf and lynx, we used 13 new STRs. We performed sequencing on a NovaSeq platform (2x150 bp) (libraries with id DIVJA088, DIVJA089, DAB074) and on a Miniseq platform (2x150 bp) (library LF22). Amplicons were sequenced in multiplexes, and necessary information for demultiplexing is in .ngsfilter files.
创建时间:
2023-11-12



