Sex dependent gene expression in human pluripotent stem cells. Homo sapiens

During early development before gonadal differentiation, sex chromosomes are the main difference between males and females. We examined any genetically driven sex dimorphisms in human pluripotent stem cells focusing on Y chromosome contribution. In order to understand the consequence of these differences, human ES cells were differentiated into EBs under the influence of Estrone (E1) and the global expression diffrences between male and females were examined. Overall design: Male and female ES cells were differentiated by EB formation under E1 for RNA extraction and hybridization on Affymetrix GENE ST 1.0 microarrays (GPL6244). RNA was extracted at 0d 3d and 11d