Effects of over-expression of PLEK2 on gene expression in 22RV1 cells

We previously found that loss of Plek2 decreased Akt activity and reverted disease phenotypes in hematopoietic system specific Pten knockout mice. Pten knockout mice also show prostate cancer phenotype. Using a well known Pten prostate-specific knockout mouse model, we found that loss of Plek2 also reverted tumorigenesis of prostate cancer via decreasing Akt pathway activity. Furthermore, we found that PLEK2 over-expression increased AKT pathway activity and promoted proliferation in 22RV1 cells which is a kind of prostate cancer cell line. To explore additional pathway which PLEK2 may involve in prostate cancer, we performed RNA sequencing in PLEK2 over-expressed and control 22RV1 cells. Overall design: To explore additional pathway PLEK2 involving in prostate cancer, we performed PLEK2 over-expression (OV) in 22RV1 cells. Total RNAs were extracted from PLEK2 OV and control groups, followed by RNA sequencing, in triplicate, using Hiseq4000 1x 50bp platform.