Sexual reproduction is controlled by successive transcriptomic waves in Podospora anserina

A whole genome microarray approach has been engaged in the heterothallic euascomycete Podospora anserina to identify genes that regulate the key steps of its sexual development. A time-course transcriptional study of 10 developmental stages from fertilization to ascospore maturation has been performed. More than half of the genome (58% of the 10540 CDS) displayed a significant change of transcript levels between at least two developmental stages of the kinetic (i.e. on all comparisons between the 10 developmental stages). With GPL10116 : reference design with a WT strain. Three independant biological replicates, the common reference is a pool of four conditions M48h, M96h, C48h and C96h ; Conditions are labelled in Cy3 and the common reference in Cy5,