Figure_4_replicates

<em>(B, F, G) HEK293T ishXrn1cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing insertions of the indicated lengths from the </em>YKT6<em> or </em>STOML2<em> genes (B). In F and G, constructs also carry mutations that disrupt the hairpin structure and restore with a different sequence. PR8 PA-X or an empty vector were also co-transfected. RNA was extracted to run 5’ RACE. DNA bands were purified and sequenced to confirm their identities.</em> <em>R# --&gt; replicate number; AI#/LG### --&gt; experiment number.</em> <em>.sgd are the original source files acquired with the Syngene Imager.</em>