supplementary data

The supplementary material provides comprehensive raw data for the mycelial growth inhibition of 30 isolates under two different fungicide treatments.​Table S1: Records the radial growth (mm) of isolates when treated with the multisite protectant Tridex Super 75 WG at concentrations of 25, 50, 75, and 100 ppm. It demonstrates a uniform, steep decline in growth across all strains.​Table S2: Details the response to Difenoconazole, highlighting the distinct subgroup of resistant isolates (e.g., TUAa51 and TUAa69) that maintained significant growth even at high concentrations.​Statistical and Phylogenetic VisualizationsAdvanced statistical modeling and validation plots are included to illustrate the differences in fungicide performance.​Figures S1–S4: Contain mixed-effects model plots, correlation matrices between chemical treatments, and Tukey’s HSD post-hoc comparison charts. Cohen’s d effect size plots further quantify the superior efficacy of Tridex over Difenoconazole at specific dose levels.​Figure S6: Presents a multiple sequence alignment of the CYP51 gene, marking specific non-synonymous mutations (e.g., Met185Leu, Asn462Lys) that differentiate sensitive wild-type strains from resistant isolates.​Structural Bioinformatics ValidationThe document includes high-resolution validation data for the homology models used in the docking simulations.​Table S3: Lists the homology modeling parameters, including high GMQE scores (0.93) and sequence identity, which confirm the reliability of the models.​Figure S5: Features a Ramachandran plot generated by PROCHECK, confirming that the vast majority of amino acid residues in the refined CYP51 models fall within the most favored and additionally allowed regions, ensuring stereochemical accuracy for subsequent docking analysis.​