Supplemental Data for "Whole-genome sequencing bulked-segregant analysis uncovered a Fusarium wilt resistance gene masked by epistasis in octoploid strawberry"
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Supplemental Data for the Article: "Whole-genome sequencing bulked-segregant analysis uncovered a Fusarium wilt resistance gene masked by epistasis in octoploid strawberry" Supplemental File S1. Fusarium wilt race 1 resistance scores for 350 ’Earliglow’ S1 n = 350 individuals recorded on seven different dates six to 12 weeks post‐inoculationSupplemental File S2. 50K array SNP genotypes (k = 49, 483) for an ’Earliglow’ S1 family (n = 327).Supplemental File S3. Fusarium wilt race 1 resistance scores for 109 ’Earliglow’ S2 individuals recorded on four different dates six to 12 weeks post‐inoculation.Supplemental File S4. Genotypic data for ’Earliglow’ S2 n = 90 progeny and m = 8, 925 variants after filtering from the FanaSNP 50K Array.Supplemental File S5. Genotypic data for ’Earliglow’ S2 progeny from the bulked‐segregant analysis in VCF format including data from four bulks with m = 5, 279, 933 variants. Supplemental File S6. Allele-specific forward and common reverse primer sequences for KASP markers targeting SNPs associated with the FW6 locus on chromosome 2B. The physical positions reported for these SNPs are in the 'Royal Royce' genome.Supplemental File S7. Genome assembly statistics for ’Royal Royce’,’ Earliglow1’, and ’Earliglow2’ genomes. Supplemental Figure S1. Number of genetic variants discovered between DNA sequence bulks of Fusarium wilt resistant and susceptible S_2 individuals across the Earliglow genome. Supplemental Figure S2. Allele frequency differences for genetic variants identified between DNA sequence bulks of Fusarium wilt resistant and susceptible S_2 individuals across the Earliglow genome. Supplemental Figure S3. Synteny among genes in Royal Royce, Earliglow-R, and Earliglow-S genomic segments spanning the FW7 locus. 'Royal Royce' gene identifiers are shown for the 12 candidate disease resistance genes documented in Table 5. Supplemental Figure S4. Predicted protein domains of FW7 candidate genes. NCBI Short Read Archive (SRA) Bioproject #PRJNA1251051: SRR33145761, SRR33145762, SRR33145763, SRR33145764, SRR33145765, SRR33145766, SRR33145767, SRR33145768, SRR33145769 NCBI Short Read Archive (SRA) Bioproject # PRJNA1253726. Both Earliglow haplotypes.



