Effect of depletion of p53 and BACH1 on gene expression in human tongue squamous cell carcinoma cell line Cal-33.

To investigate how BACH1 and p53-R175H regulate metastasis-related genes, we transiently transfected Cal-33 cells with control or p53 or BACH1 siRNA to knock down these genes expression. We then performed gene expression profiling analysis using data obtained from RNA-seq of these knockdown cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Cal-33 cells and its KD derivatives (p53 knockdown and BACH1 knockdown).