RNA sequencing analysis for mouse ovarian cancer cells and tumors response to B7-H3 (Cd276) depletion

Analysis of mouse ovarian cancer cell lines HM-1 and ID8, and HM-1 tumors inoculated to B6C3F1 mice with B7-H3 (Cd276) knockout (KO) by CRISPR-Cas9. We identified B7-H3 expression is upregulated in IFN-?–low, PD-L1–low non-immunoreactive tumors of high grade serous ovarian cancer. We explored the influence of B7-H3 on immune evasion outside of its direct regulatory function on target cells by generating B7-H3 knockout cell lines and tumors in syngeneic mouse models. Overall design: Total RNA from HM-1-B7-H3 KO cells, ID8-B7-H3 KO cells or their control cells, or intradermal tumors of HM-1-B7-H3 KO cells or their control cells at day 10 of tumor growth were extracted and used for RNA sequencing. Differential expression analysis was performed using DESeq2 (RRID: SCR_015687) between control (n = 3) and B7-H3 KO (n = 3) groups of the ID8 and HM-1 cell lines, and HM-1 intradermal tumors.