The epigenetics and non-coding RNA regulation of the heterogeneities and fates of the pluripotent stem cells. Mus musculus

This project takes early embryonic development in humans and mice, and in vitro differentiation of human and mouse pluripotent stem cells as models and research objects, and uses the existing and developing single-cell high-throughput sequencing technology to systematically analyze the gene expression, epigenetics, post-transcriptional regulation, and RNA structural heterogeneity in the process differentiation in vivo and in vitro. With the advanced bioinformatic algorithms, especially deep learning, we want to identify new pluripotent stem cell fate determination process intermediate cell states, discover new rare cell subpopulations, propose new concepts and theories, and focus on analyzing the key regulatory mechanisms and regulatory networks related to epigenetic factors, RNA binding proteins, and non-coding RNAs behind stem cell heterogeneity. In addition, we want to research on the functions and mechanisms of key regulatory factors and networks with advanced technologies such as genome editing to manipulate key regulatory factors.