Next generation high throughput sequencing to evaluate bacterial communities in freshwater ecosystem of hydroelectric reservoirs. Fresh water

The quality of aquatic ecosystems is of major public health concern. The assessment and management of a freshwater system can employ the ecological monitoring of the microorganisms present in it as indicators of both the environment and the water quality standard for protection of human and animal health. It is well known that the microbiological contamination with bacteria associated to anthropogenic activities is one of the major challenges for monitoring of the surface water status within rivers and freshwater reservoirs. Therefore, understanding the composition of aquatic microbial communities can be beneficial to early detection of pathogens, improving our knowledge on their ecological niches, and characterizing the assemblages of microbiota responsible for degradation of contaminants and microbial substrates. The present study aimed to characterize the bacterial microbiota of water samples collected alongside the Madeira River in rural areas in the vicinity of the Santo Antonio Energia (SAE) reservoir in the municipality of Porto Velho, Rondonia state, Western Brazil. A metagenomic approach was employed using Illumina NGS of the V4 region of the 16s rRNA, and physicochemical characteristics of the water sampled.