Specific activities of purified GSTE7 with alternative substrates.

The data are means ± SD of 3 replicate measurements and the background activities were corrected by using the same concentration of solvent without enzyme. Specific activities of purified GSTE7 with a

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Assay conditions for the specific activity determination with alternative substrates.

All measurements were performed in 0.1 M sodium phosphate buffer, pH 6.5 at 30°C. The stock solutions for Isothiocyanates were prepared in acetonitrile (2% final concentration in the assay), and that

Figshare2015-12-02 更新00

Lc-p75 activity on purified muropeptides selected as substrates.

aDi, disaccharide dipeptide; Tri, disaccharide tripeptide (L-Ala-D-iGln-L-Lys); Tetra, disaccharide tetrapeptide (L-Ala-D-iGln-L-Lys-D-Ala); Disaccharide, GlcNAc-MurNAc; iGln, isoglutamine; N, Asn; Ac

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