Effects of dopamine on HIV latently-infected U1 macrophage cell line. Effects of dopamine on HIV latently-infected U1 macrophage cell line

U1 monocytic cell line was cloned from U937 cells that survived an infection with Human Immunodeficiency Virus HIV-1 and bear integration sites, serving as one of the most studied HIV latent models. Under the hypothesis that the hyperdopaminergic environment of the brain of substance users affects HIV-infected cells and latency, we tested the effects of Dopamine and two dopamine receptors that were identified as able to signal changes ininnate immune cells, DRD1 and DRD4. Overall design: U1 cells were exposed for 24 hrs to Dopamine 1uM, SKF 38393 (1uM DRD1 selective agonist), and PD 168077 (1uM DRD4 selective agonist). The concentrations were pre-determined in dose-response experiments, and