Transcriptomic and Chromatin accessibility profiling of functional brown adipocytes derived from human pluripotent stem cells. Transcriptomic and Chromatin accessibility profiling of functional brown adipocytes derived from human pluripotent stem cells

Brown adipocytes (BAs) are a potential therapeutic cell source for the treatment of metabolic disease such as type 2 diabetes. In this report, human pluripotent stem cells (hPSCs) are subject to directed differentiation to brown dipocytes through a paraxial mesoderm intermediate at high-efficiency. RNA-Seq and ATAC-seq was performed to characterized hPSCs derived paraxial mesoderm and brown adipocytes generated in this study. Overall design: RNA-Seq was perfomed on iPSC and hESC derived paraxial mesoderm, iPSC and hESC derived full differentiated brown adipocytes. The differentiation process of brown adipocytes was also interrogated by RNA-Seq profiling at various differentiation time points. Time-course ATAC-Seq profiling was perfomed to interrogate the early differentiation process from paraxial mesoderm to brown adipocytes at early differentiation stages.