Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads. Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads

The goal of this study was to determine whether there was a direct relationship between stress and DNA methylation changes in fish gonads. During sex differentiation, we studied the sensitive window to density. Sex ratios were studied and methylation of canonical reproduction-related genes was analysed. Overall design: Zebrafish were reared at high density conditions as described in Ribas et al., 2017 J. Exp. Biol. During 7-21 days post fertilization (dpf) and 18-45 dpf. At 90 dpf gonads were dissected and flash frozen with liquid nitrogen for further molecular analysis and sex ratios were recorded